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apc cy7 cd48 hm48 1 cell surface markers  (Miltenyi Biotec)


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    Miltenyi Biotec apc cy7 cd48 hm48 1 cell surface markers
    Apc Cy7 Cd48 Hm48 1 Cell Surface Markers, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd48+apc/CD48+Antibody%2C+anti-mouse/10__1172_slash_jci191713-301-20-12
    Average 93 stars, based on 51 article reviews
    apc cy7 cd48 hm48 1 cell surface markers - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Fluorescence:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Comparison:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Colony-forming Unit Assay:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Purification:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Two Tailed Test:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Injection:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Control:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Isolation:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Transplantation Assay:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.

    Cell Culture:

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines.
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included the following: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
    Article Snippet: block (BD Pharmingen) for 5 min on ice, cells were incubated for 20 min on ice using staining buffer (PBS with 4% fetal bovine serum and 0.4% EDTA). .. Antibodies used included: CD14-FitC (Miltenyi Biotec), CD85-PEvio770 (Miltenyi Biotec), CD172a-APC (Miltenyi Biotec), CD97-PEvio770 (Miltenyi Biotec), CD31-PE (Miltenyi Biotec), CD366-PEvio615 (Miltenyi Biotec), CD62L-APC (Miltenyi Biotec), CD58-PE (Miltenyi Biotec), CD191-PEvio770 (Miltenyi Biotec), CD52-PEvio615 (Miltenyi Biotec), CD48-APC (Miltenyi Biotec). .. Cells were analyzed in a BD FACSCanto-II flow cytometer.

    Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells
    Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec). ... We used the following antibodies; Sca1-APC-Cy7 (1:100, 108126, Biolegend, Paris, France), Sca1-VB-FITC (1:100, 130-116-490, Miltenyi Biotec), CD48-PE-Cy7 (1:100, 560731, BD Biosciences, Le Pont de Claix, France), CD48-FITC (1:100, 103404, Biolegend), CD48-APC (1:100, 130-124-727, Miltenyi Biotec), c-Kit-BV650 (1:100, 135125, Biolegend), c-Kit-PE-Cy7 (1:100, 558163, BD Biosciences), CD150-PerCP-Cy5.5 (1:100, 115921, Biolegend), murine CD45.2-Vioblue (1:100, 130-102-980, Miltenyi Biotec).. AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.AF555-conjugated cholera toxin subunit B (CTB, 1 μg/mL, C-34776, Thermo Fisher Scientific) were used to stain lipid rafts.



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    Image Search Results


    (A) Genotyping analysis of Myd88 WT ;Rosa-CreERT2 and MyD88 L252P ;RosaCreERT2 homozygous mice following 4-OHT induced recombiation. ( B ) Proportions of donor-derived CD45.2 CD11b+Gr1lo and CD11b+Grhi cells in the PB of Myd88 WT and Myd88 L252P on left panel. Error bars represent SEM (n = 11-13 per group). (C) Representative flow cytometry gating of donor-derived CD45.2 CD11b+Gr1lo and CD11b+Grhi cells in the PB of Myd88 WT or Myd88 L252P . Error bars represent SEM. Significance was determine with a Student’s t-test for two groups or ANOVA for multiple groups (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) Representative flow cytometry gating of donor-derived CD45.1 and CD45.2 LK (Lin-cKit+Sca1-), LSK (Lin-ckit+Sca1+), LT-HSC (LSK CD150+CD48-), ST-HSC (LSK CD150-CD48-), MPP (LSK CD150-CD48+), CMP (LK CD34+16/32-), MEP (LK CD34-CD16/32-), GMP (LK CD34+CD16/32+), CLP (Lin-ckit-Sca1loCD127+CD135+) from the BM of mice competitively engafted with Myd88 WT or Myd88 L252P BM cells. (E) Representative images of spleens and BM sections (Hematoxylin and Eosin) from recipient mice transplanted with Myd88 WT and Myd88 L252P BM cells.

    Journal: bioRxiv

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion

    doi: 10.1101/2025.06.19.660202

    Figure Lengend Snippet: (A) Genotyping analysis of Myd88 WT ;Rosa-CreERT2 and MyD88 L252P ;RosaCreERT2 homozygous mice following 4-OHT induced recombiation. ( B ) Proportions of donor-derived CD45.2 CD11b+Gr1lo and CD11b+Grhi cells in the PB of Myd88 WT and Myd88 L252P on left panel. Error bars represent SEM (n = 11-13 per group). (C) Representative flow cytometry gating of donor-derived CD45.2 CD11b+Gr1lo and CD11b+Grhi cells in the PB of Myd88 WT or Myd88 L252P . Error bars represent SEM. Significance was determine with a Student’s t-test for two groups or ANOVA for multiple groups (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) Representative flow cytometry gating of donor-derived CD45.1 and CD45.2 LK (Lin-cKit+Sca1-), LSK (Lin-ckit+Sca1+), LT-HSC (LSK CD150+CD48-), ST-HSC (LSK CD150-CD48-), MPP (LSK CD150-CD48+), CMP (LK CD34+16/32-), MEP (LK CD34-CD16/32-), GMP (LK CD34+CD16/32+), CLP (Lin-ckit-Sca1loCD127+CD135+) from the BM of mice competitively engafted with Myd88 WT or Myd88 L252P BM cells. (E) Representative images of spleens and BM sections (Hematoxylin and Eosin) from recipient mice transplanted with Myd88 WT and Myd88 L252P BM cells.

    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Techniques: Derivative Assay, Flow Cytometry

    ( A ) 4-OHT (1 μM) treated cKit+ enriched BM cells (n = 1000) from Myd88 WT and Myd88 L252P mice were plated in methylcellulose and assessed for colony formation (n = 3 per group from biological replicates): erythroid progenitor cells (BFU-E), granulocyte-macrophage progenitors (CFU-G/M/GM), and multi-potential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). ( B ) Serial colony replating potential of cKit-enriched BM cells from MYD88 WT and MYD88 L252P mice in methylcellulose. Error bars represent the SEM (n = 3 per group from biological replicates). ( C ) Representative images of colonies from panel B. (C) Experimental overview of competitive BM transplants (cBMT). ( E ) Summary of donor-derived Myd88 WT and Myd88 L252P PB proportions (CD45.2) from the cBMT recipient mice at the indicated time points (n = 14-15 mice per group). ( F ) Representative flow cytometry plots of donor-derived CD45.1 (WT) or CD45.2 (Myd88 WT or Myd88 L252P ) PB cells from primary cBMTs. ( G ) Proportions of donor-derived CD45.2 populations from the PB of primary cBMTs: myeloid (CD11b + ), T (CD3 + ), and B (B220 + ) cells at the indicated time points (n= 11-13 mice per group). Error bars represent SEM (n = 11-13 per group). ( H ) Proportions of donor-derived CD45.2 populations from the BM of primary cBMTs at 16 weeks (n = 7-10 mice per group): LK (Lin - cKit + Sca1 - ), LSK (Lin - ckit + Sca1 + ), LT-HSC (LSK CD150 + CD48 - ), ST-HSC (LSK CD150 - CD48 - ), MPP (LSK CD150 - CD48 + ), CMP (LK CD34 + 16/32 - ), MEP (LK CD34 - CD16/32 - ), GMP (LK CD34 + CD16/32 + ), CLP (Lin - ckit - Sca1 lo CD127 + CD135 + ). ( I ) Experimental overview of non-competitive BM transplants (BMT). ( J ) Complete PB counts of MYD88 WT and MYD88 L252P at the indicated time points post BM transplantation and tamoxifen administration. Error bars represent SEM (n = 7-8 mice per group). ( K ) Kaplan-Meier survival curves for recipient mice transplanted with MYD88 WT (n = 12) and MYD88 L252P BM cells (n = 13 mice per group). Data from 2 independent biological replicates. ( L ) Weight of spleen isolated from recipient mice transplanted with Myd88 WT and Myd88 L252P BM cells. Error bars represent SEM (n = 7 mice per group). ( M ) Representative images of spleen sections (Hematoxylin and Eosin), PB smears (Wright-Giemsa), and lungs (Hematoxylin and Eosin) from recipient mice transplanted with Myd88 WT and Myd88 L252P BM cells. White arrows denote infiltrates of monocytes or neutrophils in the spleen. Scale bars of H&E and Wright-Giemsa images represent 50 μm. Error bars represent SEM. Significance was determined with a Student’s t-test for two groups or ANOVA for multiple groups (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

    Journal: bioRxiv

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion

    doi: 10.1101/2025.06.19.660202

    Figure Lengend Snippet: ( A ) 4-OHT (1 μM) treated cKit+ enriched BM cells (n = 1000) from Myd88 WT and Myd88 L252P mice were plated in methylcellulose and assessed for colony formation (n = 3 per group from biological replicates): erythroid progenitor cells (BFU-E), granulocyte-macrophage progenitors (CFU-G/M/GM), and multi-potential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). ( B ) Serial colony replating potential of cKit-enriched BM cells from MYD88 WT and MYD88 L252P mice in methylcellulose. Error bars represent the SEM (n = 3 per group from biological replicates). ( C ) Representative images of colonies from panel B. (C) Experimental overview of competitive BM transplants (cBMT). ( E ) Summary of donor-derived Myd88 WT and Myd88 L252P PB proportions (CD45.2) from the cBMT recipient mice at the indicated time points (n = 14-15 mice per group). ( F ) Representative flow cytometry plots of donor-derived CD45.1 (WT) or CD45.2 (Myd88 WT or Myd88 L252P ) PB cells from primary cBMTs. ( G ) Proportions of donor-derived CD45.2 populations from the PB of primary cBMTs: myeloid (CD11b + ), T (CD3 + ), and B (B220 + ) cells at the indicated time points (n= 11-13 mice per group). Error bars represent SEM (n = 11-13 per group). ( H ) Proportions of donor-derived CD45.2 populations from the BM of primary cBMTs at 16 weeks (n = 7-10 mice per group): LK (Lin - cKit + Sca1 - ), LSK (Lin - ckit + Sca1 + ), LT-HSC (LSK CD150 + CD48 - ), ST-HSC (LSK CD150 - CD48 - ), MPP (LSK CD150 - CD48 + ), CMP (LK CD34 + 16/32 - ), MEP (LK CD34 - CD16/32 - ), GMP (LK CD34 + CD16/32 + ), CLP (Lin - ckit - Sca1 lo CD127 + CD135 + ). ( I ) Experimental overview of non-competitive BM transplants (BMT). ( J ) Complete PB counts of MYD88 WT and MYD88 L252P at the indicated time points post BM transplantation and tamoxifen administration. Error bars represent SEM (n = 7-8 mice per group). ( K ) Kaplan-Meier survival curves for recipient mice transplanted with MYD88 WT (n = 12) and MYD88 L252P BM cells (n = 13 mice per group). Data from 2 independent biological replicates. ( L ) Weight of spleen isolated from recipient mice transplanted with Myd88 WT and Myd88 L252P BM cells. Error bars represent SEM (n = 7 mice per group). ( M ) Representative images of spleen sections (Hematoxylin and Eosin), PB smears (Wright-Giemsa), and lungs (Hematoxylin and Eosin) from recipient mice transplanted with Myd88 WT and Myd88 L252P BM cells. White arrows denote infiltrates of monocytes or neutrophils in the spleen. Scale bars of H&E and Wright-Giemsa images represent 50 μm. Error bars represent SEM. Significance was determined with a Student’s t-test for two groups or ANOVA for multiple groups (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Techniques: Derivative Assay, Flow Cytometry, Transplantation Assay, Isolation

    (A) BM cells were harvested, and cKit+ cells were enriched by magnetic selection. Cells were analyzed by 10X single-cell RNA (scRNA) sequencing. UMAP of 10,000 cells representing 13 clusters from recipient mice transplanted with Myd88 WT or Myd88 L252P BM collected at 12 weeks. Pseudotime trajectory of scRNA-seq profiles of the major clusters as defined by the indicated genes. (C) Immunoblotting of Myd88 WT and Myd88 L252P cKit+ BM cells incubated with 4-OHT to induce recombination for 72 hours followed by treatment with vehicle (DMSO) or 1 μM IRAK1/4-inhibitor (IRAK1/4-inh; NCGC-1481) for 24 hours. (C) Proportion of donor-derived Myd88 WT (n = 5) and (C) Myd88 L252P (n = 5) CD45.2 populations from the BM of cBMTs following treatment with vehicle or IRAK1/4-inh: LK (Lin-cKit+Sca1-), LSK (Lin-ckit+Sca1+), LT-HSC (LSK CD150+CD48-), ST-HSC (LSK CD150-CD48-), MPP (LSK CD150-CD48+), CMP (LK CD34+16/32-), MEP (LK CD34-CD16/32-), GMP (LK CD34+CD16/32+), CLP (Lin-ckit-Sca1loCD127+CD135+). Data is representative from 2 independent biological replicates. Error bars represent SEM. Significance was determined with a Student’s t-test for two groups or ANOVA for multiple groups (*, P<.05; **, P < 0.01).

    Journal: bioRxiv

    Article Title: MYD88 mutations in clonal hematopoiesis promote inflammation and hematopoietic stem cell expansion

    doi: 10.1101/2025.06.19.660202

    Figure Lengend Snippet: (A) BM cells were harvested, and cKit+ cells were enriched by magnetic selection. Cells were analyzed by 10X single-cell RNA (scRNA) sequencing. UMAP of 10,000 cells representing 13 clusters from recipient mice transplanted with Myd88 WT or Myd88 L252P BM collected at 12 weeks. Pseudotime trajectory of scRNA-seq profiles of the major clusters as defined by the indicated genes. (C) Immunoblotting of Myd88 WT and Myd88 L252P cKit+ BM cells incubated with 4-OHT to induce recombination for 72 hours followed by treatment with vehicle (DMSO) or 1 μM IRAK1/4-inhibitor (IRAK1/4-inh; NCGC-1481) for 24 hours. (C) Proportion of donor-derived Myd88 WT (n = 5) and (C) Myd88 L252P (n = 5) CD45.2 populations from the BM of cBMTs following treatment with vehicle or IRAK1/4-inh: LK (Lin-cKit+Sca1-), LSK (Lin-ckit+Sca1+), LT-HSC (LSK CD150+CD48-), ST-HSC (LSK CD150-CD48-), MPP (LSK CD150-CD48+), CMP (LK CD34+16/32-), MEP (LK CD34-CD16/32-), GMP (LK CD34+CD16/32+), CLP (Lin-ckit-Sca1loCD127+CD135+). Data is representative from 2 independent biological replicates. Error bars represent SEM. Significance was determined with a Student’s t-test for two groups or ANOVA for multiple groups (*, P<.05; **, P < 0.01).

    Article Snippet: For flow cytometric analysis of lineage positive cells or stem/progenitor HSCs, PB or BM samples were processed in 1xRBC lysis for 15 minutes, followed by incubation in the following antibodies, DAPI (D1306, ThermoFisher Scientific), 7AAD (00-6993-50, eBiosciences), CD11b-PE-Cy7 (25-0112-81, eBiosciences), Gr1-eFluor450 (48-5931-82, eBiosciences), CD3-PE (12-0031-83, eBiosciences), B220-APC (17-0452-82, eBiosciences), CD45.1-Brilliant Violet 510 (110741, BioLegend), CD48-APC (11-0481-85, eBiosciences), CD117-APC-Cy7 (135135, BioLegend), Ly-6A/E(Sca-1)-PE (12-5981-82, eBiosciences), CD135-PE-Cy5 (135312, BioLegend), CD150-PerCpCy5.5 (115922, BioLegend), CD127-Brilliant Violet 605 (35041, BioLegend) and CD45.2-APC-eFluor780 (47-0454-82, eBiosciences) or CD45.2-eFluor450 (48-0454-82, eBiosciences).

    Techniques: Selection, Sequencing, Western Blot, Incubation, Derivative Assay

    Journal: STAR Protocols

    Article Title: Protocol for the isolation and characterization of murine hematopoietic stem and progenitor cell-derived extracellular vesicles

    doi: 10.1016/j.xpro.2025.103778

    Figure Lengend Snippet:

    Article Snippet: CD48-APC (dilution 1:100) , eBioscience , Cat# 17-0481-82; RRID: AB_469408.

    Techniques: Magnetic Beads, Recombinant, Control, Isolation, Software